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WATANABE K; KAWAI M; OBATAKE Y. 1994 FRUITING BODY FORMATION OF LYOPHYLLUM-SHIMEJI IN PURE CULTURES. MOKUZAI GAKKAISHI 40: 879-882. Address: WATANABE K, NARA PREFECTURAL FOREST EXPT STN,TAKATORI,NARA 63501,JAPAN
Fruiting body formation Lyophyllum shimeji (Kawan.) Hongo in pure cultures was attempted using a soil based medium which contained commercial soil for gardening, rice-bran, and yeast extract. To incubate the fungus for a long period, the water content of the medium was adjusted to approximately 50%. After an incubation of 146-228 days, the fully colonized media were moved to a fruiting room constantly controlled at 17-degrees-C. Six days later in the fruiting room, innumerable primordia were formed on the surfaces of the media. Some of the primordia continued growing to be immatured fruiting bodies. Seven of eight stocks were capable of forming primordia, and five of these continued growing to be fruiting bodies. The long-term incubation on soil based medium probably enabled the fungus to form primordia and fruiting bodies. It was shown that the mycorrhizal fungus, L. shimeji, can form fruiting bodies independently of host plants.
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