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Zonation of vesicular-arbuscular mycorrhizal fungi along a saline gradient on three coastal sand-dunes in Japan

For Poster or Talk (4. Biodiversity and Ecology)
ABE, JUN-ICHI P.1 & K. KATSUYA1
1Institute of Agriculture and Forestry, University of Tsukuba, Tsukuba, Ibaraki 305, Japan

ICOM1 Abstract
Salinity is one of the environmental factors which has a strong influence on the zonal distribution of plants in coastal sand-dunes. The distribution of vesicular-arbuscular mycorrhizal fungi (VAMF) were examined from shoreline to inland along a saline gradient. Two study sites along the coast of the Pacific Ocean and one on the Sea of Japan differing in vegetation were chosen. Sand samples were collected from 4-6 sampling points at each line transects. Sampling was carried out from uncovered sand-dunes below the edge of the vegetation up to 190 m far from the shoreline. Three genera and 9-10 species of VAMF were isolated and identified at the three study sites. Many common species (up to 89%) were observed between the study sites. The frequency of occurrence of VAMF spores in sand samples were low on uncovered seaward sides and increased towards the landward sides. However the total number of spores per samples were higher in covered sand dunes near the vegetation limits. Scutellospora gregaria and Acaulospora sp. 1 were predominantly isolated and their frequency of occurrence increased in samples collected further inland. Glomus sp. 2 was predominantly present on covered sand-dunes near the shoreline and Glomus sp. 1 and G. aggregatum were predominantly isolated from the landward sides. These results showed evidence for zonation of Glomus spp. on coastal sand dunes, which appeared to be dependent on the saline gradient rather than on the zonation of sand-dune plants.