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Are early nodulins genes express during endomycorrhizal
symbiosis?
talk/poster
session 5
ALBRECHT CATHERINE, RENE GEURTS, KATHARINA PAWLOWSKI, AB
VAN KAMMEN, TON BISSELING
Molecular Biology, Agricultural University, Dreijenlaan 3,
6703 HA Wageningen, The Netherlands
ICOM1 Abstract
Legume mutants have been generated that are unable to form
nodules in symbiosis with rhizobia, a subset of wich are
also unable to enter mycorrhizal symbiosis. The frequent
coincidence of the Nod- and Myc- phenotype suggests that
general symbiotic genes allow plants to live with
endosymbiotic partners. To further investigate whether
development of endomycorrhizal and Rhizobium symbiosis
share common functions we have examined induction of some
early nodulins genes during the establishment of
endomycorrhiza. For this purpose, specific primers for the
early nodulins genes, ENOD 5 and ENOD 12, have been used in
a RT-PCR strategy to detect a gene expression in wild type
and mutants pea roots colonized with the endomycorrhizal
fungus Gigaspora margarita.