ICOM1 Abstract
session number: 1 Physiology or 6 Structure and Anatomy
ALLAWAY, WILLIAM G.1 & ANNE E. ASHFORD2. 1School of Biological Sciences
A12, The University of Sydney, NSW 2006, Australia. 2School of
Biological Science, The University of New South Wales, Sydney, NSW 2052,
Australia. - Choice of technique as an influence on perceptions of
apoplasmic pathways, localization of small molecules and fine structure
in mycorrhizas.
The influence on physiological interpretation due to microscopical
preparation techniques, including chemical fixation, dehydration and
embedding, precipitation methods, and cryofixation and freeze-
substitution, is discussed. Problems including changes in structure,
diffusion from in vivo locations, binding to tissue components, and
extraction by solvents, are considered. It is concluded that
physiological interpretations of images and localizations obtained with
microscopic techniques require rigorous experimental investigation of
the effects of these techniques.