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Characterization by molecualr methods (ITS-RFLP) of the
different Suillus species of the mediterranean region
poster
ANTA, BERTA1, MARIO HONRUBIA2,
CATALINA CARRILLO2, ALMUDENA
GUTIERREZ2 & VICTOR RUBIO1
1Centro Nacional de Biotecnolog’a (CSIC-UAM), E-
28049 Madrid, Spain
2Dpto. Biologia Vegetal (Botanica). Facultad de
Biologia. Universidad de Murcia. Campus de Espinardo 30100,
Murcia, Spain
ICOM1 Abstract
The relatedness of 17 isolates of Suillus , belonging to
six typical species from the mediterranean region (S.
bellini, S. collinitus, S. granulatus, S. luteus, S.
mediterranensis and S. variegatus ) and other isolate of
Suillus granulatus from Kenya, was determined comparing the
ITS region. The amplification of the ITS region with the
universal primers ITS 1 and ITS 4 produces a fragment of
constant size in all strains. The digestion of these
fragments with different restriction enymes, first with Alu
I and Sau 3A, reults in a common pattern for almost every
strain; second with the combination of Taq I, Msp I and
Hinf I identifies the different species, except of Suillus
mediterranensis. Intraespecific variation was observed in
all the species. Data presented do not support the
maintenance of Suillus mediterranensis and Suillus
collinitus as different species. Results indicate that PCR-
RFLP can be used as a simple method for ecological studies
of the Suillus species in this semiarid area in which they
are predominant. Finally we present the realtionship of
these Suillus with Suillus grevillei, Suillus spraguei and
Rhizopogon subacaurelescens (from the data base) in a
phylogenetic tree.