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Axenic growth and sporulation of Glomus intraradices from a
transformed carrot root grown in the proximity of a
pre-colonized sweet potato root from an aeroponic
system
poster
ASIF, M & ABDUL G. KHAN
Department of Biological Sciences, The University of
Western Sydney - Macarthur, P. O. Box 555, Campbelltown NSW
2560, Australia
ICOM1 Abstract
Aeroponically grown sweet potato roots infected with Glomus
intraradices were surface-disinfected and placed on a
minimal medium with or without a non-infected Ri-T DNA
transformed carrot root. Extensive mycelium and spores
developed on both the plates, although the growth was more
profuse on the plate with the transformed root, which also
became infected. When subcultured on minimal medium,
without any root segment, axenic growth occurred on the
plates inoculated for 6 weeks in dark at 25oC. The fungus
possessed aseptate mycelium with angular projections
typical of VAM fungi. Successful vesicular infection
developed in the roots of Sudan grass when inoculated with
infected hairy roots or a small agar block with axenic
fungal growth only as sources of inoculum. No arbuscules
were observed. Inoculated plants produced significantly
higher PDW than the controls but compared to plants
inoculated with sand culture, PWD were not significantly
different. However, when both sources of inoculum were
used collectively, a significant and perhaps synergistic
effect on PDW was observed.