ICOM1 Abstract
CHINNERY, LOUIS E. & LYNDON F. ROBERTSON. Department of Biology, The
University of the West Indies, P.O.B. 64, Bridgetown, Barbados. - Using
phytometers to assess the mycorrhizal potential of soils
Soils were sampled from each of the ten soil associations of Barbados.
At each site two subsamples were placed, with as little disturbance as
possible, into 15cm plant pots and a third subsample bagged for
subsequent spore extraction. Three seedlings each of corn (Zea mays),
pepper (Capsicum annuum) and bean (Phaseolus vulgaris), germinated in
autoclave sterilised growth media (soil:sand:vermiculite), were
transplanted into one set of soil samples and 24hr pre-soaked corn
achenes planted in the second set. Vesicular-arbuscular mycorrhizal
(VAM) fungal colonisation of roots was determined 2, 3 and 4 wk after
transplanting. Spore populations were low and varied between 1.02 and
2.63 g-1 soil with the lowest populations being found in the
agriculturally most productive soil type. VAM colonisation increased
with time and corn plants showed the greatest level of colonisation.
Significant differences between VAM fungal colonisation of phytometer
roots between soil types was closely related to spore density. This
study has not shown that the use of phytometers provides a better
assessment of the VAM potential of soils than counting spores.