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Researches on specific plant protein expression in arbuscular
mycorrhizal symbioses
TALK (preference) or POSTER (list afternoon contributed
session): 5
DUMAS-GAUDOT, ELIANE1, BARBARA DASSI1,
SOPHIE SLEZACK1, ASSEM SAMRA1, MARIA
JOSE POZO2 & SILVIO GIANINAZZI1
1Laboratoire de Phytoparasitologie INRA/CNRS,
SGAP, INRA, BV 1540, 21034 Dijon Cedex, France
2Departemento de Microbiologia, CSIC, Estacion
Experimental del Zaidin, 18008 Granada, Espagna
ICOM1 Abstract
Targeted and untargeted approaches have been used in several
plant species (tomato, pea and tobacco) to look for changes
in plant protein expression linked to arbuscular mycorrhizal
(AM) symbioses, pathogenic infections and pathogen-
mycorrhizal root interactions.
The targeted approach, selected to study modifications in
plant hydrolytic enzymes following arbuscular symbiosis,
pathogenic infections, or chemical elicitation, allowed us to
demonstrate, as a general phenomenon, the existence of
mycorrhiza-induced specific chitinase isoforms, probably of
host origin. The expression of several groups of Pathogenesis
Related (PR) proteins was investigated by immunological
detection (Western blotting) in plant roots inoculated with
AM and fungal root pathogens or during bioprotection in roots
pre-colonized with an AM fungus and post-infected with a root
pathogen. While the AM symbiosis more often leads to weak
expression of PR proteins compared to that in pathogenic root
interactions, a different situation occurs during
bioprotection.
An untargeted approach, using 2D-PAGE to analyze protein
content of mycorrhizal roots, has permitted to demonstrate
the existence of endomycorrhizins. This result has been
further supported by in vivo and in vitro protein synthesis
analyses. Studies by 2D-PAGE are in progress for identifying
polypeptides specifically expressed during bioprotection in
pathogen-challenged mycorrhizal roots.