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Dumas-Gaudot, E; Valot, B; Bestel-Corre, G; Recorbet, G; St-Arnaud, M; Fontaine, B; Dieu, M; Raes, M; Saravanan, RS; Gianinazzi, S. 2004. Proteomics as a way to identify extra-radicular fungal proteins from Glomus intraradices - RiT-DNA carrot root mycorrhizas FEMS MICROBIOLOGY LETTERS. 48(3):401-411. Address: Dumas-Gaudot, E, CNRS, UMR 1088, INRA, CMSE, 5184 UB,BP 86510, F-21065 Dijon, France
To identify fungal proteins involved in the arbuscular mycorrhizal symbiosis, root-inducing transferred-DNA transformed roots of carrot (Daucus carota L.) were in vitro inoculated with Glomus intraradices. Proteins extracted from the extra-radical fungus were analysed by two-dimensional gel electrophoresis. A fungal reference map displaying 438 spots was set up. Four proteins, among the 14 selected for tandem mass spectrometry analysis, were identified including a NmrA-like protein, an oxido-reductase, a heat-shock protein and an ATP synthase beta mitochondrial precursor. The possible fungal Origin of a MYK15-like protein found in mycorrhizal roots was further discussed. This is the first report of arbuscular mycorrhizal fungal protein identifications by using a proteomic approach.
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