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Agueda, B; Parlade, J; Fernandez-Toiran, LM; Cisneros, O; de Miguel, AM; Modrego, MP; Martinez-Pena, F; Pera, J. 2008. Mycorrhizal synthesis between Boletus edulis species complex and rockroses (Cistus sp.). MYCORRHIZA. 18(8):443-449. Address: Agueda, B, Consejeria Medio Ambiente, Dept Invest & Experiencias Forestales Valonsadero, Junta Castilla & Leon,Apdo Correos 175, Soria 42080, Spain
Ectomycorrhizas of Boletus aereus, Boletus edulis, and Boletus reticulatus were synthesized with Cistus sp. under laboratory conditions using synthesis tubes filled with a mixture of sterilized peat-vermiculite and nutrient solution. The fungal strains isolated from sporocarps were identified by molecular techniques. The inoculated seedlings were grown for 4-5 months. The ectomycorrhizas formed were described based on standard morphological and anatomical characters. The three ectomycorrhizas described were very similar, with white monopodial-pinnate morphology, a three-layered plectenchymatous mantle on plan view and boletoid rhizomorphs.
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