Kumar, T; Majumdar, A; Das, P; Sarafis, V; Ghose, M. 2008. Trypan blue as a fluorochrome for confocal laser scanning microscopy of arbuscular mycorrhizae in three mangroves. BIOTECHNIC & HISTOCHEMISTRY. 83(3-4):153-159.
Kumar, T, Indian Stat Inst, Agr & Ecol Res Unit, 203 BT Rd, Calcutta
Roots of three mangroves, Acanthus ilicifolius, Ceriops tagal and
Excoecaria agallocha, collected from forests of the Sundarbans of India
were stained with trypan blue to observe arbuscular mycorrhizal
colonization. Spores of arbuscular mycorrhizal fungi isolated from
rhizospheric soil, collected together with the root samples, also were
stained for testing the suitability of the dye as a fluorochrome.
Confocal laser scanning microscopy images were constructed. A.
ilicifolius and E. agallocha exhibited Arum type colonization with
highly branched arbuscules, whereas C. tagal showed Paris type
association with clumped and collapsed arbuscules. We demonstrated that
trypan blue is a suitable fluorochrome for staining arbuscular
mycorrhizal fungal spores, fungal hyphae, arbuscules and vesicles,
which presumably have a considerable amount of surface chitin. It
appears that as the integration of chitin into the fungal cell wall
changes, its accessibility to trypan blue dye also changes.