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Blaszkowski, J; Kovacs, GM; Balazs, T. 2009. Glomus perpusillum, a new arbuscular mycorrhizal fungus. MYCOLOGIA. 101(2):247-255.
A new arbuscular mycorrhizal fungal species of genus Glomus, G.
perpusillum (Glomeromycota) I forming small, hyaline spores is
described and illustrated. Spores of G. perpusillum. were formed ill
hypogeous aggregates and occasionally inside roots. They are globose to
subglobose, (10-)24(-30) pin diam, rarely egg-shaped, oblong to
irregular, 18-25 X 25-63 mu m. The single spore wall of G. perpusillum,
consists of two permanent layers: a finely laminate, semiflexible to
rigid outer layer and a flexible to semiflexible inner layer. The inner
layer becomes plastic and frequently contracts in Spores crushed in
PVLG-based mountants and stains reddish white to grayish red in
Melzer's reagent. Glomus perpusillum. was associated with roots of
Ammophila arenaria, colonizing sand dunes of the Mediterranean Sea
adjacent: to Calambrone, Italy, and this is the only site Of its
occurrence known to date. In single-species cultures with Plantago
lanceolata as host plant, G. perpusillum formed vesicular-arbuscular
mycorrhiza. Phylogenetic analyses of partial SSU sequences of nrDNA
placed the species in Glomus group A with no affinity to its subgroups.
The sequences of G. perpusillum unambiguously separated from the
sequences of described Glomus species and formed a distinct clade
together with in planta arbuscular mycorrhizal fungal sequences found
in alpine plants.
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