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Fu ShaoChun; Tan Qi; Chen MingJie; Shang XiaoDong; Cai LingYi; Zhang MeiYan. 2009. Mycorrhizal synthesis involving Boletus edulis and Pinus massoniana. Acta Edulis Fungi. 16(1):31-41. Mycorrhizae involving Boletus edulis and Pinus massoniana have been successfully established and their morphological characteristics described. Optimal conditions for mycorrhizal synthesis were determined using orthogonal testing of three parameters: substrate, age of host plant and inoculation procedure. Following integration analysis of plant survival rates, diameter of seedling rootstock, height of seedling shoot, number of mycorrhizae, ratio of shoot/root biomass, number of mycorrhizae, and the ratio of infected/non-infected root tips, we concluded that a double-layered growth substrate that simulated natural soil structure was superior for mycorrhizal synthesis compared with the more commonly used single-layered peat moss and vermiculite substrate, especially when sepiolite powder was also added.
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